Chris Perani’s Extreme Macro Photography of Butterfly Wings

Nature can be very surprising sometimes, and in this case, it is the beauty of the very complex and detailed Butterfly Wings we are looking at. American photographer Chris Perani specializes in Extreme Macro using microscope objectives to photograph insects. His High Definition Range (HDR) process consists of 2,100 separate exposures of a butterfly wing merged into a single photo. To photograph their wings he use a 10x microscope objective attached to a 200mm lens.
Since Perani is using a microscope objective, the depth of field is almost nonexistent. Using a focus rail, the lens must be moved no more than 3 microns per photo to achieve focus across the thickness of the subject which can be up to 8 millimeters. This yields 350 exposures, each with a sliver in focus, that must be composited together. This process yields one piece of 6 piece puzzle. The process is repeated 6 times for different sections of the wing with the final result being the composite of these pieces.